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In this study, we built a yeast strain that produces the sucrose invertase Suc2p upon illumination with light and tested the population growth dynamics and the benefits of cooperation in various artificially shaped landscapes of cooperators and cheaters. The OptoSuc2 strain indeed acts as a cooperating cell when illuminated but remains a cheater when kept in the dark because non-illuminated cells cannot metabolize sucrose and instead rely on the hexose produced by adjacent cooperating cells. We were, therefore, able to quantitatively explore the spatial metabolic interactions between cooperators and cheaters. Nature — Optogenetic spatial patterning of cooperation in yeast populations | Nature Communications
AtSWEET4 coding sequence was cloned into vector pDR196, the resulting construct was introduced into yeast hexose transport mutant EBY.VW4000 strain41. The yeast transformants were first grown on selective synthetic complete medium (without uracil) with 2% maltose as the sole carbon source. Drop tests were used to assess the growth of transformed yeast in 2% glucose and 1% fructose solid SD media with a serial of dilutions (OD500 = 1, 10−1, 10−2, 10−3, 10−4). The transformants with empty vector was used as a negative control. All transformants were grown at 30 °C for 3 days. Nature — AtSWEET4, a hexose facilitator, mediates sugar transport to axial sinks and affects plant development | Scientific Reports
To address this problem, we studied the interaction between five variants of hCK2α (WT protein and four histidine mutants) and three reference ionizable bromo-benzotriazoles and their conditionally non-ionizable benzimidazole counterparts (see Fig. 2) to identify the contribution of long-range electrostatic interactions involving the particular histidine residue to ligand binding. Nature — Effect of histidine protonation state on ligand binding at the ATP-binding site of human protein kinase CK2 | Scientific Reports
Shilatifard, A. The COMPASS family of histone H3K4 methylases: mechanisms of regulation in development and disease pathogenesis. Annu. Rev. Biochem. 81, 65–95 (2012). Nature — Early life changes in histone landscape protect against age-associated amyloid toxicities through HSF-1-dependent regulation of lipid metabolism | Nature Aging
Robart, A. R. & Collins, K. Investigation of human telomerase holoenzyme assembly, activity, and processivity using disease-linked subunit variants. J. Biol. Chem. 285, 4375–4386 (2010). Nature — Structure of human telomerase holoenzyme with bound telomeric DNA | Nature
I thought of him as a renaissance man as he crunched numbers and understood the human spirit effortlessly together. It was he who would, in defining moments, acknowledge a piece of advice as homespun wisdom and proceed to resolve the issue with that in mind, parallel with the data. BusinessMirror — Homespun wisdom | Ritzi Villarico-Ronquillo
The Hawaiian Lending & Investments (HLI) nonprofit founded by the Sovereign Council of Hawaiian Homestead Associations (SCHHA) has partnered with the Department of Hawaiian Home Lands (DHHL) to support home building and home ownership by Native Hawaiians waiting for a homestead lot award. | Homestead nonprofit partners with DHHL to serve waitlisted Hawaiians — Homestead nonprofit partners with DHHL to serve waitlisted Hawaiians : Maui Now
If you’ve recently acquired a home in Florida and miss the homestead exemption filing deadline, it could cost you thousands. All Floridians should be aware of the homestead exemption and the Save Our Homes amendment. As the March 1 annual filing deadline approaches, many homeowners who qualify for these significant tax breaks risk missing out. New homeowners in Florida should get acquainted with what the laws are, and how they are administered. Tampa Bay Times — Deadline approaches for taking of advantage of Florida’s homestead exemption
Li, G., Yu, W. & Cui, Y. A homochiral nanotubular crystalline framework of metallomacrocycles for enantioselective recognition and separation. J. Am. Chem. Soc. 130, 4582–4583 (2008). Nature — Free-standing homochiral 2D monolayers by exfoliation of molecular crystals | Nature
Future work will focus on design structurally diverse chiral POMOFs and optimizing such reactions. The structural simplicity of catalyst and the use of inexpensive and readily available chemical reagents render this approach highly promising for the development of practical homochiral materials for asymmetric catalytic reactions. Nature — Polyoxometalate-based homochiral metal-organic frameworks for tandem asymmetric transformation of cyclic carbonates from olefins | Nature Communications
Study of homochiral self-sorting. a Illustration that the crossed C−I···S halogen bonds between one L,L-IA and one D,D-IA molecules are unlikely because of the mismatched orientation of I-atom and S-atom. b Plots of CD signal at 291 nm of IA in CH3CN against ee. [L,L-IA] + [D,D-IA] = 5 μM. c Partial 850 MHz 1H NMR spectra of –CH3h in L,L-IA, D,D-IA and their racemate in CD3CN at 25 °C. The solutions were sample saturated Nature — Single-handed supramolecular double helix of homochiral bis(N-amidothiourea) supported by double crossed C−I···S halogen bonds | Nature Communications
Emphasizing the importance of skill development in improving employability of youth, Nirmalanandanatha Swami of Adichunchangiri Mutt has said that by honing their abilities through skill training, youth will gain a competitive edge over their peers and increase their chances of securing employment. The Hindu — Honing abilities through skill training will help increase chances of landing jobs, youth told - The Hindu
The show, which runs from 11 am to 6 pm on Friday and from 9:30 am to 3:30 pm on Saturday (with the last entry at 2:30 pm), promises an array of vibrant displays showcasing the best of local horticultural achievements. Among the participants are the dedicated gardeners from St. Michael’s Mount, who are excited to present their finest blooms alongside other exceptional specimens grown in the fertile soils of West Cornwall. Cornish Stuff — West Cornwall Spring Show Celebrates 100 Years of Horticultural Excellence - Cornish Stuff
Action News got a preview Friday morning for the nation's largest and longest-running horticultural event. 6abc Philadelphia — Philadelphia Flower Show 2024: Nations largest horticultural event blooms at the Pennsylvania Convention Center - 6abc Philadelphia
[REPLAY] 'Ai hotu #31 : fish and chips uru sauce tartare, le gombo et l'astuce recyclage Polynésie la 1ère — [REPLAY] 'Ai hotu #31 : fish and chips uru sauce tartare, le gombo et l'astuce recyclage - Polynésie la 1ère
This unique blend of Hollywood glamour and British heritage promises to bring a new perspective to the Isle of Wight, potentially inspiring fans and tourists to explore this charming destination. As the hovercraft journey unfolds, it will be interesting to see how this star-powered expedition further connects the worlds of cinema, history, and adventure. BNN — Florence Pugh Plans Unique Hovercraft Trip with Zendaya to Isle of Wight Dunes
A MAJOR RNLI and coastguard operation saw a helicopter and a hovercraft launched after a person was reported “walking out onto the mud flats” off Southend. Echo — Southend RNLI and coastguard launch helicopter and hovercraft | Echo
The Montreal Gazette reported that a $3,500 cockatoo was taken from another Montreal store in June of 1988 and a $15,000 hyacinthine macaw was stolen in Beloeil, Que., in July — the latter bird was recovered, however, after it was too hard for the alleged thieves to sell. CBC — The $50,000 bird heist that left feathers in the air duct | CBC
Schopka, H. H., Gudmundsson, M. T. & Tuffen, H. The formation of Helgafell, southwest Iceland, a monogenetic subglacial hyaloclastite ridge: Sedimentology, hydrology and volcano-ice interaction. J. Volcanol. Geothermal Res. 152, 359–377 (2006). Nature — Pyroclastic passage zones in glaciovolcanic sequences | Nature Communications
Magnús Tumi finds it interesting how the lava fills up valleys and depressions and flows around the hyaloclastite [i.e. formed in a subglacial eruption] mountains. Weakling Grown Strong: Eruption that Surprises - Iceland Monitor